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2015 Volume 6 Issue 2

Laccase production by Aspergillus niveus on SSF using wheat bran as alternative carbon source and its synergistic effect on pulp biobleaching using a mix of laccase/xylanase from the same microorganism


Abstract
Aiming to investigate the production oflaccases by Aspergillus niveus RP05, the performance of this enzyme on cellulose pulp biobleaching wasanalyzed and tests of mixing laccase/xylanases from the same microorganism were also included. A.niveus was cultivated at 30ºC, for 2 weeks on solid state fermentation (SSF) composed by wheat bran and 0.05 M (NH4)2HPO4 (1:2 – w/v) for laccase production. Optima of temperature and pH reaction were 60ºC and 4.5 – 7.0, respectively. The correlation between pH and temperature was investigated using the experimental design and the highest enzyme activity levels were obtained at the central points (55°C, pH 5.0). It was verified that only the temperature had significance in the linear level with the confidence interval of 95%.The enzyme was stable up to 70ºC (more than 80% of residual activity); after 1 hour of incubation laccase lost at least 50% of its initial activity from 75 – 80ºC. Crude extract containing laccase activity itself was used (STRATEGY I) or mixed with xylanase (STRATEGY II) to clarify cellulose pulp. The most interesting result was obtained with a mix of xylanase/laccase, resulting in Kappa efficiency around 56% and an increase in whiteness on the order of 17.2 points.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative