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2012 Volume 4 Issue 1

Kinetic analysis of the thermostability of peroxidase from African oil bean (Pentaclethra macrophylla Benth) seeds


Abstract
A major problem in the storage and marketing of processed African oil bean seeds is its high deterioration rates due to the undesirable activity of peroxidases. The effect of heat treatment on the activity of peroxidase (POD) from African oil bean (Pentaclethra macrophylla) seeds was studied over a range of 30°C to 80°C. A simple first-order reaction which assumes a biphasic thermoinactivation curves was used to study the denaturation of this enzyme. Results suggested that peroxidase is a stable enzyme with a Z-value as low as 0.0147. This indicates that it is more sensitive to increase in temperature than to the duration of the heat treatment making high temperature short time treatment a technique to be used for its inactivation. The results of the thermodynamic investigations indicated that the oxidation reactions were: (a) not spontaneous (ΔG > 0) for peroxidase at 323oK, and at all the temperatures (2) slightly endothermic (ΔH > 0) at 323 K and (3) reversible (ΔS < 0) at all the temperatures under study.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative