In the automotive industry, using artificial colors has contaminated natural ecosystems such as groundwater and living organisms. Vehicle colors are one of the most dangerous pollutants of the environment. Biodegradation and using bacteria in order to reduce paint contamination is a cost-effective method, and it is a favorable method for the environment. Therefore, screening of decomposing bacteria of colored compositions from contaminated soils by automotive color was carried out in Fars province. In this study, 12 strains of negative and positive gram bacteria were isolated. Using diagnostic kits, identification of isolates indicated that they included bacterial species such as Streptomyces and bacilli. In order to evaluate the efficacy of these isolates, it was done in liquid culture medium in colorless condition and with the absorbance rate of 260 nm (at 1 to 24 hours); by using the spectrophotometer and in the presence of white metallic and oily paints, isolates were examined on the 6th, 9th and 12th days. The highest percentage of strains was Streptomyces that was classified as the degrading bacteria for oily and metallic paints (91.66%, 81.66%) respectively; and the least percentage of strains was bacillus species (5.15%, 7.33%) respectively. Based on the percentage of bleaching, the most color degradation was observed at 30 ° C and PH7. The data show that the conditions of the culture medium can have a great impact on the growth and color degradation by the color degrading bacteria.