Introduction and goal: In obstructive azoosperm, a person has normal spermatogenesis but no sperm is ejaculated due to obstruction in the sperm from the testes to the ejaculation. Also the disorder of the expression of each of the effective genes in the pathway of spermatogenesis is one of the causes of azoospermia one of these genes being SCP3 and producing a protein in the phase of homologous and synaptic chromosomes. The lack of expression of this gene in mice causes male infertility and reduced fertility of the female sex. Examination of this gene in humans can also help determine the cause of infertility and as a diagnostic factor in determining the progress of spermatogenesis and infertility in the treatment of male patients. Methods: In this descriptive cross-sectional study, the expression of Scp3 gene in healthy and control tissues of men with obstructive visophagus referred to Ebncenna and Mrs. Al-Nabiya infertility in 1396 1397 was evaluated and compared by real time polymerase chain reaction (Real time PCR). Results: In order to determine the gene expression in obstructive oesophageal men which according to our research hypotheses that the absence of this gene causes impaired spermatogenesis and fertility of the individual, the relative level of expression in the control tissue with healthy tissue was the same and no significant changes were observed. Conclusion: In this study, the SCP3 gene was not expressed in patients with obstructive oesophageal disease which made them suitable molecular markers for determining the presence of sperm and examination of spermatogenesis in infertile men without requiring invasive testicular biopsy.
