The aim of this study was investigating the effectiveness of acceptance and commitment therapy (ACT) on self-compassion and psychological well-being of the experienced-infidelity women referring to the counseling centers of Shiraz, Iran, in the second half of 2018. The present research design was quasi-experimental with the control group through the analysis of covariance method. The research population included all the experienced-infidelity women in Shiraz that were sampled by purposive volunteer sampling. Among the experienced-infidelity women referring to 4 counseling centers of Shiraz, some were volunteer to participate in the study, thus 30 experienced-infidelity women were selected as the clinical sample and were assigned into two equal groups of experimental and control. Neff’s self-compassion Scale (SCS) and Ryff's scales of psychological well-being (SPWB) (short-form) were chosen as the tools of the study. The experimental group undergone 8 sessions of 90-minute intervention based on the acceptance and commitment therapy (ACT), but the control group did not received any intervention. The analyses of the inferential statistics of multivariate analysis of covariance (MANCOVA) and analysis of covariance (ANCOVA) were used to analyze the data. Statistical analyses were carried out by SPSS, version 25, software. The results of MANCOVA and ANCOVA indicated that the intervention being used caused the increase of self-compassion and psychological well-being of the experienced-infidelity women. Generally, it can be claimed that the intervention of acceptance and commitment therapy (ACT) on the experienced-infidelity women was effective and is highly recommended.