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2019 Volume 10 Issue 3

Improvement of Characteristics of Maltase from Marrow (Cucurbita pepo L.) Cotyledons by its Immobilization on Chitin


Salem A. Habib, Adel I. Al-Alawy, Hamed M. El-Shora, Mohamed I. Sakran, Mohammed A. H. Al-Duais and et al
Abstract

Maltase is one of the key enzymes in our digestive processes, as it is the main salivary enzyme in the digestion of carbohydrates in the mouth. The present investigation aimed to evaluate the impact of some additive materials on maltase activity, and the role of immobilization of the enzyme on chitin in improving its characters. After different purification steps, the recorded specific activity of maltase from Cucurbita pepo L. was 385 units mg-1 protein and the fold was 4.4. Addition of EDTA, 0.5% (w/v) bovine serum albumin (BSA), 10 mM nicotinic acid, 20 to 30 mM PEG, glycine, proline, and alanine stimulated the maltase activity. On the other hand, 0.5 % (V/V) of Triton–X100, Tween-80, 10 mM diphenylamine, and 5% (v/v) 2-mercaptoethanol inhibited maltase activity. Also, maltase activity was inhibited by succinic anhydride > sodium fluoride >phethalic anhydride in the order as arranged. After 5 days of storage at 25 ℃, the enzyme retained 43.5% of its activity. After immobilization on chitin, the enzyme retained 162.4 U from 240 U of its activity after 6 cycles of reusability. Also, the pH and temperature of optimum activity were shifted from 6.0 and 50 ℃ in case of free maltase to 9.0 and 60 ᴼC in case of immobilized maltase respectively. Also, K+, Mn2+, Mg2+, Co2+, and Zn2+ activated immobilized maltase with a higher rate compared with the free maltase. On the other hand, Hg2+ and Cu2+ inhibited the immobilized maltase activity at a lower rate than free maltase. Moreover, immobilized maltase expressed higher response to activation with sorbitol and mannitol than free maltase‎‎.


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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative