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2020 Volume 11 Issue 2

Hydrothermal and Calcination Regimes and Characteristics of Nanohydroxyapatite Synthesized from Salmon bones


Nguyen Tri, Tran N. D. Trang, Nguyen H. D. Trinh, Lai T. Tung, Nguyen T. T. Van, Nguyen P. Anh, Nguyen D. Tan, Nguyen T. H. No, Huynh K. P Ha
Abstract

Salmon bones - byproducts in food industries, was regarded as waste and will be discarded without fully utilizing it. From the approach of waste to wealth in the chemical compositions, the by-product can be employed as a cheap bioresource to form nanohydroxyapatite. In this paper, the influence of hydrothermal and calcined conditions on the structure of nano-hydroxyapatite synthesized from the salmon fishbone by the hydrothermal method was investigated. Various techniques such as X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and X-ray fluorescence spectrometry (XRF) were used to characterize the samples. XRD studies revealed that hydrothermal and calcined conditions affected the crystallinity as well as the purity of the hydroxyapatite. The suitable conditions for the synthesis of nanohydroxyapatite were proposed such as the hydrothermal at 120 °C for 8 hrs and calcined at 800 °C for 2 hrs. At the conditions, the hydroxyapatite had a spherical shape with the nanoparticle size in a range of 30–60 nm, and reached a BET surface area of 13.8 m2.g−1, a  pore volume of 0.018 cm3.g−1, and a pore diameter of 24.8 Å. The molar ratio of Ca/P in the hydroxyapatite was 1.64, which is the ratio one would expect for hydroxyapatite.


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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

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new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative