HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax

If there would be free heavy metals occurring in the organismwithout control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures as nucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC)from GSH by phytochelatin synthase. PC´s are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500μM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5.