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2009 Volume 1 Issue 4

Horseradish peroxidase catalyzed free radical cannot free move in reaction solution


Abstract
Mechanism of Horseradish Peroxidase -catalyzed phenol compound oxidizing reaction is a radical polymerization. Many polymer preparation are also carry on through the radical polymerization mechanism We deduce if free radical produced by peroxidasecatalyzed phenol polymerization could apply on polymer preparation? Could the phenol–oxygen free radical leave off the peroxidase and catalyze other compounds polymerization? The free radical in phenol oxidation process was investigated in homogeneous reaction and in immobilized HRP catalyzed reaction. The results showed the free radical produced by peroxidase only move on the surface of enzyme, can’t free move in solution in both experiment. Evidence showed the phenol polymerization is enzyme reaction process, different from general chemistry free radical chain reaction.Keywords: Horseradish Peroxidase, free radical polymerization, mechanismReceived: 17 March 2008 / Received in revised form: 5 February 2009, Accepted: 31 April 2009 Published online: 14 May 2009

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

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Welcome to the Journal of Biochemical Technology, a prestigious international double-blind peer-reviewed journal dedicated to advancing excellence in biochemical, biotechnological, and bioinformatics research with direct applications in biology and medicine.

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The Journal of Biochemical Technology is dedicated to publishing innovative research in the fields of biochemistry, biotechnology, and bioinformatics with direct applications in biology and medicine.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative