Agrotechnical methods of grain-crop cultivation require chemical substances to protect the plants from dangerous pests and diseases. However, these measures lead to soil deterioration due to the fungicides and pesticides accumulating in the soil. One of the promising and effective techniques to reduce the deterioration and combat pathogens is using growth regulators. The paper presents the results of a study into the effects of the Krezatsin (concentration of 15, 30 and 60 mg/l), Zircon (concentrations of 0.1, 1 and 10 mg/l) and Appin (concentration of 0.1, 1 and 10 mg/l) growth regulators on the mycelial growth of Fusarium sporotrichioides and Fusarium сulmorum. Screening of the regulators’ effect on the F. culmorum and F. sporotrichoides strains has demonstrated that only Cresacin (concentration of 15 mg/l) and Zircon (all the concentrations) can consistently inhibit mycelial growth, so these growth regulators can be recommended as an effective means to protect grain crops from Fusarium L.