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2012 Volume 4 Issue 1

Genic microsatellite markers for genetic diversity of rust resistant wheat genotypes


Abstract
The genetic diversity and molecular characterization of 10 wheatgenotypes was investigated using 27 polymorphic microsatellitescreened primers including Simple Sequence Repeats (SSR). Abouttwenty one loci were found. Bioinformatics tools were applied forconstructing dendogram. Lr-19 gene was present in all ten wheatgenotypes and Sr-15 gene is present in nine genotypes except Uqab-2000 that cause resistance against wheat rust. Highest geneticdiversity was observed between Shalimar-86 and Pak-81 genotypesand showed the similarity of about 95.34%. Distantly related Uqab-2000 showed minimum genetic diversity and 65.64% dissimilaritywith Kohistan-97. Uqab-2000 rust resistant genes may have aninsertion or deletion and examined as distantly related to rest of ninegenotypes. The current research found that SSR makers identifiedrust resistant genes in numerous wheat genotypes. Present work alsocharacterized the wheat genotypes at molecular level and foundgenetic diversity between different wheat genotypes. SSR markerscould distinguish and characterize wheat genotypes, more screenedprimers could be used for saturation of different regions in furtherresearch. Bioinformatics also play a vital role in retrieving,analyzing and interpreting the data for further studies.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative