In order to investigate the effect of drought stress or dry crop on biochemical properties of some advanced lentil genotypes, the experiment was conducted as split plots based on randomized complete block design with three replications and in dry and low irrigation conditions as the main factor and genotype As a subsidiary in 2 areas, Ardabil Agricultural Research Station and the central part of Germi city were cultivated in the years 2015-2016. The biochemical traits included proline accumulation, cytoplasmic membrane stability index, percentage of relative water content of the cell, peroxidase enzyme, ascorbate peroxidase enzyme, soluble sugars, phenolic compounds and quantitative trait including grain yield.Combined analysis of variance and mean comparison showed the significant difference between rainfed and low irrigation cultivation in most evaluated characteristics; as though, phenolic compounds, solved sugars, and ascorbate peroxidase enzyme were the highest amounts in rained irrigation condition. Seed performance in rainfed condition was 22% less than low irrigation. A and b chlorophyll and proline amounts were more in Germi region, but peroxidase and ascorbate peroxidase enzymes had the maximum amounts in low irrigation condition in Ardabil. The maximum solved sugar, peroxidase enzyme, cytoplasmic membrane stability, and proline were related to genotype 1, 3, 4, and 12 showing varieties and selectivity of cultivars for resistance against low irrigation The correlation between proline and peroxidase enzyme was positive and significant. In addition, the correlation between seed performance with cytoplasmic membrane stability, cell relative water content, ascorbate peroxidase enzymes and phenolic compounds was significant and positive.