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2015 Volume 6 Issue 3

Enhanced lipase production of Fusarium verticillioides by using response surface methodology and wastewater pretreatment application


Abstract
Lipases are enzymes able to hydrolyze the ester bond of long-chain acylglycerols. This work aimed to investigate the production, optimization and characterization of Fusarium verticillioides lipase and its application on fatty acid production and fat particles hydrolysis. Response surface methodology (RSM) was employed to optimize the production. Adams medium was used in the submerged fermentation, at 30°C for 96h. Sunflower oil and peptone were the best carbon and nitrogen sources. The optimized medium showed an activity 2-fold higher than the original medium and was composed by 0.15 % KH2PO4, 0.025 % MgSO4, 0.3 % peptone and 1 % sunflower oil. The optimal temperature and pH was 45°C and 5.5, respectively. The enzyme had a remarkable stability in a pH range of 4.0-8.0. The hydrolysis of vegetable oils showed sunflower oil as better hydrolyzed compared to others. A fat reduction of 3-fold was evaluated in the slaughterhouse pretreatment.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative