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2015 Volume 6 Issue 3

Enhanced biodecolorization of textile dye Remazol navy blue using an isolated bacterial strain Bacillus pumilus HKG212 under improved culture conditions


Abstract
Biodegradation of synthetic dyes has been proven as the most efficient, eco-friendly and cost-effective alternative for the treatment of textile dyes contaminated waste water. Remazole Navy Blue (RNB), an azo dye used extensively in the commercial textile industry constitutes hazardous recalcitrant, when released in the aqueous environment. In this research, an isolated bacterial strain identified as Bacillus pumilus HKG212 was studied for its potential to decolorize RNB dye. The isolated bacterial strain showed significant decolorizing capacity of more than 95% up to an initial dye concentration of 500 mg/L, within 48 hours and was efficiently tolerant up to a higher concentration of 1500 mg/L, under static condition. We investigated various physicochemical parameters to achieve maximum dye degradation by Bacillus pumilus HKG212.  The optimal conditions for the decolorizing activity of Bacillus pumilus HKG212 were anaerobic culture environment with 10% innoculm volume and beef extract as a nitrogen source, at pH 8.0, and 30℃.  Biodegradation of RNB dye was confirmed through UV-VIS Spectrophotometer, HPLC and FTIR analysis. Our findings confer the potential application of strain Bacillus pumilus HKG212 for decolorization of RNB dye and hence treatment of industrial wastewaters containing dye pollutants. 

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative