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2023 Volume 14 Issue 3

Effect of Pimpinella anisum and Salvia officinalis Extracts on Developing Liver of Chick Embryo: 14-Day Incubation


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Abstract

The present study was designed to evaluate the effects of P. anisum and S. officinalis extracts on the liver of chicken embryos. Eighty fertilized chicken eggs were divided into eight groups.  The first group (C) served as a control group. The second, third, and fourth groups (P1, P2, and P3) of high, medium, and low concentrations of P. anisum aquatic extracts. The fifth, sixth, and seventh groups (S1, S2, and S3) of high, medium, and low concentrations of S. officinalis aquatic extracts. The eighth group (M) of medium concentrations of P. anisum and S.officinalis aquatic extracts. All groups were injected in ovo (0.1 ml/egg) of aquatic extracts before incubation and the eighth day of incubation. After 14 days of incubation, the eggs were opened and biochemical assays in liver homogenate were made. Also, histological studies were made in the liver. Results revealed that the activity of liver homogenate levels of SOD, GSH, and CAT significantly decreased in P1, S1, and M-treated chicken embryos, while significantly increasing in P2, P3, S2, and S3-treated chicken embryos. A significant increase in liver homogenate levels of MDA was observed in all treatment groups. Histological studies of the liver in medium and low concentrations showed normal structures. Furthermore, the liver sections in P1, S1, and M-treated chicken embryos showed some damage. In conclusion, high concentrations of P. anisum and S.officinalis and the combined group have negative effects on the embryo chick liver. While medium and low concentrations have positive effects due to their antioxidant constituents.


How to cite this article
Vancouver
Al-Ghamdi FA, Aljarari RM, Albalawi NS. Effect of Pimpinella anisum and Salvia officinalis Extracts on Developing Liver of Chick Embryo: 14-Day Incubation. J Biochem Technol. 2023;14(3):59-66. https://doi.org/10.51847/LVrdeVMDkf
APA
Al-Ghamdi, F. A., Aljarari, R. M., & Albalawi, N. S. (2023). Effect of Pimpinella anisum and Salvia officinalis Extracts on Developing Liver of Chick Embryo: 14-Day Incubation. Journal of Biochemical Technology, 14(3), 59-66. https://doi.org/10.51847/LVrdeVMDkf
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative