The present study was designed to evaluate the effects of P. anisum and S. officinalis extracts on the liver of chicken embryos. Eighty fertilized chicken eggs were divided into eight groups. The first group (C) served as a control group. The second, third, and fourth groups (P1, P2, and P3) of high, medium, and low concentrations of P. anisum aquatic extracts. The fifth, sixth, and seventh groups (S1, S2, and S3) of high, medium, and low concentrations of S. officinalis aquatic extracts. The eighth group (M) of medium concentrations of P. anisum and S.officinalis aquatic extracts. All groups were injected in ovo (0.1 ml/egg) of aquatic extracts before incubation and the eighth day of incubation. After 14 days of incubation, the eggs were opened and biochemical assays in liver homogenate were made. Also, histological studies were made in the liver. Results revealed that the activity of liver homogenate levels of SOD, GSH, and CAT significantly decreased in P1, S1, and M-treated chicken embryos, while significantly increasing in P2, P3, S2, and S3-treated chicken embryos. A significant increase in liver homogenate levels of MDA was observed in all treatment groups. Histological studies of the liver in medium and low concentrations showed normal structures. Furthermore, the liver sections in P1, S1, and M-treated chicken embryos showed some damage. In conclusion, high concentrations of P. anisum and S.officinalis and the combined group have negative effects on the embryo chick liver. While medium and low concentrations have positive effects due to their antioxidant constituents.
