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2012 Volume 4 Issue 1

Dose response screening of free and encapsulated ellagic Acid against 7,12-Dimethylbenz(a)anthracene induced oxidative stress on hamster buccal pouch carcinogenesis


Abstract
Ellagic acid, a phenolic phytonutrient has become a focus of intense research owing to its role in prevention and treatment of cancer. In the present study, we proposed to screen the dose response effect of free ellagic acid (EA) and ellagic acid encapsulated nanoparticles (EANP) against DMBA induced oxidative stress on hamster buccal pouch model. DMBA (0.5% in mineral oil) was topically applied to the left buccal pouch of male Syrian hamsters 3 times a week for 14 weeks. Treatment groups received EA (20, 40, 80 mg/kg bw) and EANP (10, 20, 40 mg/kg bw) via oral gavage 3 times a week from 10 to 21 weeks. Animals were sacrificed at the end of the experimental period and free radical mediated oxidative damage was estimated using various biochemical markers such as lipid peroxidation and antioxidants (GSH, SOD, CAT & GPx) which are the key indicators for cancer risk at the precancerous stage. DMBA induced positive controls showed altered levels of lipid peroxidation which is associated with diminished cellular antioxidant status. Treatment with EA and EANP significantly augmented the activities of cellular antitoxidants and ultimately diminished the levels of lipid peroxidation which point towards suppression of preneoplastic lesions thereby reduces the cancerous risk.  Thus from the aforementioned results it is showed that treatment with EA at the dose of 40 mg/kg bw and EANP at the dose of 20 mg/kg bw was found to be the optimal dose which proved antioxidant activity against DMBA induced oxidative stress on hamster buccal pouch carcinogenesis.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative