Linoleic acid isomerization was performed in this study and the production of conjugated Linoleic acid was tested. Treatment groups comprised four variable temperatures (80, 110, 140 and 170 °C), four durations (0.25, 0.5, 1, 2, 3.5, 5 and 6.5 hours), four solvents (water, ethanol, propylene and glycol), and four alkalis (sodium hydroxide, potassium hydroxide, sodium methoxide and potassium methoxide). The relevant tests were aimed at identifying the best temperature, treatment period, solvent and alkali for the production of conjugated linoleic acid, which was 140 °C and 30 min. Results showed that the excessive increase in temperature caused the conversion of beneficial isomers) C9-T11 and T10-C12) to other isomers of conjugated linoleic acid and other unusual products. The excessive increase in temperature reduced the desirable product, but longer duration of time did not cause this problem. It was concluded that there is approximately a 50/50 ratio between the two isomers under optimum conditions.
