Background: While various studies have compared functional limitation and fear of movement differences between people with and without low back pain (LBP), but it is not clear what differences exist in the functional limitation and demographic variables in classified LBP people based on the movement system impairment (MSI) model. Therefore, this study was designed to compare the disability, fear avoidance and habitual physical activity between LBP subgrouped people and healthy subjects. Methods: A total of sixty women between 18 and 55 years of age voluntarily participated in this study. They were divided into three groups (20 healthy, 20 Rotation Extension (R.E), and 20 Rotation Flexion (R.F)). Participants completed self report measures including Oswestry Disability Index (ODI), Tampa Scale of Kinesiophobia (TSK) and Baecke habitual physical activity questionnaire (BHPAQ). Results: compared with the R.F subgroup, people in the R.E group reported greater level of disability and fear on movement during daily tasks (p < 0.05). Conclusion: These results may have important implications for understanding differences evaluation and treatment of people with LBP. When assessing people with and without LBP, characteristics such as activity limitation, movement behavior and LBP classification should be considered.