There are several industries which could threat our environment. Among them the textile factories by using different dyes disturbing more. Therefore, introducing the new techniques or, bacterial strains is interesting for the environmental –friendly researchers. Hence, the aim of this study is isolation of bacteria from soil samples with decolorization capability. For this purpose, 30 soil samples were collected from Marvdasht province, Iran. The samples were serially diluted and cultivated on nutrient agar media. The selected colonies were identified using biochemical tests and the percentage of decolorization was calculated. The optimum temperature and dye concentrations were evaluated and the best isolates were molecularly identified. The results obtained from this study indicated that five different strains were able to decolorize the direct blue 129. Among the isolates Bacillus licheniformis strain DSM 13 and Pseudomonas aeruginosa strain SNP0614 were the best one. The best temperature and dye concentration were 40 and 0.1g/l respectively. Overall, although different dyes using in this geographical area, identification of the native strains and spending them for wastewater treatment could help the dirty environment and reduce the amount of toxic materials.
