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2015 Volume 6 Issue 3

Biodegradation of carbazole by Pseudomonas sp. GBS.5 immobilized in polyvinyl alcohol beads


Abstract
The Pseudomonas sp. GBS.5 cells were immobilized in calcium alginate (2%): PVA (6%) matrix and the carbazole degradation was studied after dissolving the alginate linkages. This matrix was chosen for immobilization based on mechanical stability and immobilized cell viability. The maximum carbazole degradation rate achieved by PVA-alginate immobilized cells was 52.62 ppm h-1 as opposed to 39.06 ppm h-1 by cells immobilized in calcium alginate beads. The effect of cell loading capacity and initial carbazole concentration on carbazole degradation were also studied. The cells immobilized in PVA beads could be stably stored and be repeatedly used for eight cycles at 100 ppm carbazole concentration with high degradation activity. The immobilization process described in the paper resulted in increased carbazole degradation activity and hence the decrease in the cost of operations.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative