Presents the results of a long-term study of spring barley collection samples from the gene pool of VIR. Field experiments were carried out on conventional agricultural techniques. Collection samples seeds were sown in optimal time (I decade of may) on plots of 2 m2. Shoots are obtained on the 10-15th day in different years. The vegetation period of the standard in different years was from 81 to 92 days depending on weather conditions, and collection samples from 65 to 93 days. The harvest was harvested in the III decade of August. Barley collections were evaluated according to VIR guidelines and other original methods. Biochemical analysis of barley samples was carried out in the spectrometer Spectra Star 2400, the protein content is found through the determination of nitrogen by Kieldalmetod and the conversion factor N x 5, 7 and N x 6,25 for malting barley, filminess – according to the method of antispyware crops.