The objective of this study was to evaluate the variation and importance of certain hematological, enzymatic, and oxidative stress markers in women with breast cancer under chemotherapy treatment. Hematological parameters, enzymes’ activities, and oxidative stress status were estimated in cancer patients and the sensitivity and specificity of biomarkers (MDA, GSH, Catalase, and ORAC) in blood and saliva were compared between the patient and control groups using receiver operating characteristics (ROC) curve design. Results showed that the erythrocyte line (RBC, HGB, and HCT) significantly decreased (p <0.001, p <0.01, and p <0.05, respectively) and ALP, amylase, and transaminases activities were not significantly changed in breast cancer patients group compared to the control group and the reference values. The results showed that GSH levels in leukocytes, erythrocytes, and saliva, as well as ORAC in serum, were significantly increased in patients with breast cancer compared to healthy controls, whereas MDA level and catalase activity did not change significantly. On the other hand, there was not any significant correlation in GSH levels between erythrocyte, leukocyte, and saliva in patients and control groups. ROC analysis indicated that GSH and ORAC levels are better diagnostic tools than other oxidative markers, suggesting to be used as systematic markers for diagnosis and prognosis of breast cancer.