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2012 Volume 4 Issue 1

Application of factorial design to the study of xylitol production from corncob hemicellulose hydrolysate by Candida guilliermondii


Abstract
Optimization of the culture medium and process variables for xylitol production from corncob hemicelluloses hydrolysate was carried out using Candida guillermondii (NCIM 3124). The optimization was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on xylitol production was achieved using a Plackett-Burman design. The four selected components were optimized with Box-Behnken design using response surface methodology (RSM). The optimum level (g/l) is: MgSO4.7H2O- 1.34, yeast extract- 4.34, KH2PO4- 2.94 and xylose- 9.49 and influence of various process variables on the xylitol production was evaluated. The optimal levels were quantified by the central composite design using RSM. The optimum level of process variables are: temperature (29.88 oC), substrate concentration (3.26 g/l), pH (7.25), agitation speed (170.42 rpm), inoculum size (3.41 ml). These conditions were validated experimentally which revealed an enhanced xylitol yield of 0.73 g/g.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative