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2015 Volume 6 Issue 2

Anti-inflammatory activity and COX 2 inhibition potential of Trois in murine macrophage cell line


Abstract
The objective of present study was to investigate the anti-inflammatory effects of Trois in lipopolysaccharide (LPS) induced macrophage RAW 264.7 cells. A biomembrane stabilisation assay was also conducted to evaluate its role in biomembrane fluidity during inflammation. Cytotoxicity of drugs on RAW 26-4.7 macrophages cell line was conducted using 3-(4,5-dimet-hylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. mRNA expression of cyclooxygenase-2 (COX-2) was studied us-ing polymerase chain reaction (PCR). Results of our study revealed that IC50 of Trois and volini was 2.82 and 1.73 mg/ml, respectively, after 24 hrs treatment. Further, our data proved that Trois had excellent anti-inflammatory activity as it caused reduced production of nitric oxide (9-12%), myeloperoxidase (5-14%) and mRNA of COX-2 (12-33%) in comparision to volini. More-over, Trois had 5.44% more membrane stabilization potential compared to volini. From the above study it can be concluded that Trois can be an excellent option for the treatment of inflammation related diseases.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative