Irritable bowel syndrome is a functional multifactorial gastrointestinal disorder, stress is one of the proposed factors that play a crucial role in the exaggeration of (IBS). The study aimed to determine the prevalence and impact of stressful education on Irritable Bowel Syndrome among Medical and Non-Medical Students at Al-Neelain University. A cross-sectional study was carried out at Al-Neelain University faculty of Medicine and Commerce Khartoum State, Sudan, out of 270 students were chosen proportionately utilizing systematic random sampling method, Data were gathered using a pre-tested, structured questionnaire. A total of (270) students from both faculties; 44.8% (121) students of the faculty of medicine and 55.2% (149) from the faculty of accounting. The overall prevalence of IBS was 27.2% in the faculty of medicine while (26.8%) were diseased in the faculty of commerce. Among medical diseased students; IBS Diarrhea predominant was significantly 76%, IBS Mixed predominant type was 15%, and IBS Constipation 9%. We conclude that Irritable bowel syndrome is a common disease among Al-Neelain University students, more common in medical students than nonmedical ones, which can be considered as a female prevalent disease.