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2015 Volume 6 Issue 1

Partial purification and characterization of carboxyl esterase in aged and lithium treated rat brain


Abstract
Specific activity of Carboxyl esterase (CE) was estimated for young,old (2-4 and 16-18 months) and for similar groups of Lithiumtreated male Albino Sprague dawley rats whole brain cells. Rats ofboth age groups were orally administered with 37mg of Lithiumchloride (LiCl2) per kg body weight daily for 10 days. Old rat brainsLiCl2 treated showed significant increased CE specific activity of0.076 IU/mg protein in crude, 0.1445 IU/mg protein in Ammoniumsulphate precipitated and 0.5827 IU/mg protein in ion exchangefraction than their control. So the highly purified samples ofcarboxyl esterases obtained with LiCl2 treated brain. Enzymekinetics of substrate concentration showed Km of 0.2 mM andVmax of 52.631, indicating higher CE activity at pH-6 and at 60oCtemperature in crude extracts of control old rats. The crude brainsamples of both aged groups LiCl2 treated showed an intense bandpatterns in Native PAGE for CE expression than their controls.Thus, LiCl2 elevates carboxyl esterase in each step of purificationand in future it activates drug metabolism to treat various brainrelated disorders.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative