Pycnoporus coccineus and Coriolus versicolor are among the tropical white rot basidiomycetes that degrade lignin selectively. In this research work, crude enzyme produced during biopulping using both fungi were extracted, filtered and assayed using specific substrates. Both the peroxidases enzyme activities and residual lignin content were measured for the incubation period of 20, 40 and 60 days of biopulping. For both fungi, manganese peroxidase is predominant and highly expressed thus shows the highest rate compared to other ligninolytic enzymes activities in all of extract preparations. After 60 days of inoculation, manganese peroxidase activities are recorded as 270.51 U/mL and 274.36 U/mL for C. versicolor and P. coccineus, respectively. On the evaluation of the lignin content after biopulping, the lignin content showed significant decreased. Wood chip biotreated with C. versicolor showed higher percentage in lignin loss (9.42%) compared to with P. coccineus (8.10%).