Quick access

PDF
Share Article:
2016 Volume 7 Issue 3

Isolation, Screening, Characterization and Optimization of Xylanase Production from Thermostable Alkalophillic Fusarium sp. XPF5


Prachi Sharma, Neelam Kaushik, Sushma Sharma, Vinod Kumar
Abstract
Xylanases are the enzymes that hydrolyse xylans, a major constituent of hemicelluloses and have various biotechnological applications. It is produced by various microbial sources including fungi being most promising. In the present study, soil samples rich in plant dead material obtained from various locations Himalayan region around Solan, Himachal Pradesh, India, and screened for xylanase producing fungal isolates. Seven fungal isolates were selected on the basis of clear zone formation around the fungal growth for quantification of xylanase production. Among the seven fungal strains isolated, isolate XPF-5 was selected as hyper producing fungal strains showing highest enzyme activity (0.398 U/ml). The strain was identified microscopically as Fusarium sp. XPF-5 on the basis of morphological characteristics. Optimum temperature and pH for XPF-5 xylanase were found as 57⁰C and 9.0, respectively. The production of xylanase was optimized using different factors such as temperature, pH and time of incubation by ‘one variable at a time’ approach. The optimum temperature, pH and time of incubation for maximum xylanase production were obtained as 47⁰C, 8.0 and 96 h, respectively. Based on the results of culture conditions and xylanase activity, the fungal isolate XPF-5 was considered as thermo-alkalophile, and make it a potential candidate for large scale xylanase production and its use in biotechnological processes.

INDEXING
+ Pubmed Central, DOAJ, SCIRUS, BiologyBrowser, Chemical Abstracts, CABI, Intute catalogue, Science Central, EBSCOhost databases, Google Scholars, Genamics JournalSeek, Open J gate, Ulrich's, ResearchGate and many more.

J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

Publish with us

Call for papers

Issue 3 Volume 11 - 2020

Publishing steps

1.Prepare
your paper
2.Submit
and revise
3.Track
your research
4.Share
and promote
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative