This study describes the isolation and characterization of a novel bacterium for denitrification process under aerobic conditions. Twenty two denitrifying strains were isolated from sludge of secondary clarifier of waste water treatment plant of a local fertilizer industry. The isolated strains were initially screened using solid media for their denitrifying activities. Among them one of the bacterium displayed the highest reduction of nitrate without much accumulation of nitrite. The isolate was identified preliminarily by biochemical tests as Pseudomonas syringae, and further confirmed its identification by 16S rRNA sequencing analysis. This isolate strain showed 99.32% nitrate reduction under anoxic condition as well as 99.1% nitrate removal under aerobic condition.