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2012 Volume 4 Issue 2

Improvement of anaerobic production of hydrogen in the dark by genetic mutation strains of Synechocystis sp. strain PCC 6803


Abstract
The anaerobic H2 production in the dark by bidirectional hydrogenase of unicellular cyanobacterium Synechocystis sp. strain PCC 6803 has been studied. D-glucose addition to cell suspension has been known to enhance H2 production, and the two mutants, glucose tolerant strain and its mutant lacking L-lactate dehydrogenase (LDH) gene, were introduced to improve H2 production with D-glucose. Higher D-glucose uptake rate of glucose tolerant strain resulted in higher H2 production rate comparing with the wild type strain. The LDH mutant showed higher initial H2 production rate and H2 yield from D-glucose than the other strains because the NADH which should be consumed originally by LDH can be redistributed to the H2 production reaction.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative