The major objective of the study was to determine the effect of different concentrations of cadmium chloride on nutrients that are available and biochemical properties of four varieties of wheat PUNJAB-2011, MILLAT-2011, LASANI and AARI. It has been estimated that chlorophyll a, chlorophyll b and total chlorophyll contents were decreased under CdCl2 stress conditions. However, the uptake of cadmium was increased in all levels of cadmium stress (0, 50, 100 and 150 mM) in both shoots and roots (mg/g dry weight) of all cultivars. Cadmium adversely effected all the tested biochemical parameters like, chlorophyll a, b and total chlorophyll contents. Total carotenoids of wheat and the availability of necessary ions such as calcium, phosphorus, zinc and iron also decreased in the cadmium stress conditions. The extent of cadmium stress has increased and distinguished with increasing concentrations of cadmium chloride that led to having adverse effects on the yield and production of tested wheat genotypes.