Lipases are enzymes able to hydrolyze the ester bond of long-chain acylglycerols. This work aimed to investigate the production, optimization and characterization of Fusarium verticillioides lipase and its application on fatty acid production and fat particles hydrolysis. Response surface methodology (RSM) was employed to optimize the production. Adams medium was used in the submerged fermentation, at 30Â°C for 96h. Sunflower oil and peptone were the best carbon and nitrogen sources. The optimized medium showed an activity 2-fold higher than the original medium and was composed by 0.15 % KH2PO4, 0.025 % MgSO4, 0.3 % peptone and 1 % sunflower oil. The optimal temperature and pH was 45Â°C and 5.5, respectively. The enzyme had a remarkable stability in a pH range of 4.0-8.0. The hydrolysis of vegetable oils showed sunflower oil as better hydrolyzed compared to others. A fat reduction of 3-fold was evaluated in the slaughterhouse pretreatment.