Effect of PGR producing bacterial strains isolated from vermisources on germination and growth of Vigna unguiculata (L.) Walp.


Abstract

Nineteen bacterial strains were isolated from vermisources andscreened for Indole-3-acetic acid (IAA) production among themonly nine strains produce IAA and they were identified asStreptococcus spp., Micrococcus spp., Klebsiella spp., Bacillus spp., Enterobacter spp., Escherichia spp., Alcaligenes spp., Erwinia spp., and Pseudomonas spp. Among all other strains Bacillus sp. showed the higher IAA production hence selected for further molecular analysis and confirmed as Bacillus cereus. The B. cereus was grown in nutrient broth supplemented with different concentrations (1, 2, 3, 4 and 5mg/ml) of tryptophan for seven days at pH 7 and at 37ºC. Crude IAA was used for in vitro phytostimulatory studies using Vigna unguiculata (L.) Walp. The plant growth parameters were analyzed at different day intervals (5, 10 and 15 days). Supplementation of 5 ml crude IAA (2mg/ml of tryptophan) dynamically enhances the plant growth parameters after 15 days.



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