Asymmetric Bioreductionof Ketones with a Pumpkin (cucurbita)‎

Bilal Khaled , LakhdarSekhri , MessaoudaGuemmouda , Mohammed Said Nedjimi , Touhami‎ Benhachem , Fatah Benmoussa


In this study, a general, efficient, and simple methodology for biocatalytic reduction of carbonyl ‎compounds such as acetophenone 1a and 4-chloroacetophenone 2a using freshly cut ripen fruit of fresh ‎pumpkin in the aqueous medium at room temperature was reported. The prochiral ketones can be ‎reduced to chiral secondary alcohols in a generalized way. The obtained results indicated that the ‎pumpkin fruits could be used as biochemical catalysts to contribute to the preparation of many ‎pharmaceutical alcohols. This biochemical catalyst attracted much attention because of the low cost, ‎high efficiency, and special selectivity for its environmental friendliness and its contribution to certain ‎recommended green chemistry principles. ‎

In this research, our aim was to contribute to this area by using biochemical catalysts with plant ‎sources such as the pumpkin fruits in different states (fresh, juice). The prochiral ketones: ‎acetophenone and 4-chloroacetophenone were chosen as typical ketones and the yield and optical ‎purity were 77-88% and 50-96%, respectively. Mild reaction condition, simple operation, and easy ‎availability of fresh pumpkin fruit revealed this protocol as an attractive and alternative eco-friendly ‎option for a general reduction of all types of carbonyl compounds‎‎‎‎.

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