Anti-inflammatory activity and COX 2 inhibition potential of Trois in murine macrophage cell line


Abstract

The objective of present study was to investigate the anti-inflammatory effects of Trois in lipopolysaccharide (LPS) induced macrophage RAW 264.7 cells. A biomembrane stabilisation assay was also conducted to evaluate its role in biomembrane fluidity during inflammation. Cytotoxicity of drugs on RAW 26-4.7 macrophages cell line was conducted using 3-(4,5-dimet-hylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. mRNA expression of cyclooxygenase-2 (COX-2) was studied us-ing polymerase chain reaction (PCR). Results of our study revealed that IC50 of Trois and volini was 2.82 and 1.73 mg/ml, respectively, after 24 hrs treatment. Further, our data proved that Trois had excellent anti-inflammatory activity as it caused reduced production of nitric oxide (9-12%), myeloperoxidase (5-14%) and mRNA of COX-2 (12-33%) in comparision to volini. More-over, Trois had 5.44% more membrane stabilization potential compared to volini. From the above study it can be concluded that Trois can be an excellent option for the treatment of inflammation related diseases.



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