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2015 Volume 6 Issue 1

A novel psychrophilic Janthinobacterium lividum MMPP4 isolated from Manimahesh Lake of Chamba District of Himachal Pradesh, India


Abstract
We isolated psychrophilic bacterial strains from high altitude (elevation 13,390 ft) Manimahesh Lake of Chamba District of Himachal Pradesh. Based on phenotypic characteristics and growth appearance at 4ºC, four bacterial isolates (MMPP2, MMPP4, MMPP5, and MMPP7) were selected and showed optimum growth at 20ºC and no growth was detected at 25ºC.  Qualitative assay showed that cell free medium of MMPP4 prevent inhibition of freezing (antifreeze activity) as determined by freezing of cell free medium at -20ºC for seven days. Proteinase K treatment and heat inactivation at 80ºC for 6 h resulted in the loss of antifreeze activity, suggesting a proteinaceous nature of antifreeze activity. MMPP4 produces protease, phytase and lipase enzymes, but did not produce amylase. Moreover, cell free supernatant of MMPP4 showed proteinaceous type of antimicrobial activity against S. aureus as measured by agar well diffusion assay.  To identify the MMPP4 strain, 16s rDNA was amplified using 27F and 1492R standard primers and sequenced on both the strands. Nucleotide BLAST and phylogenetic analysis showed 99% identity to Janthinobacterium lividum strain and deposited in GenBank accession no. as KJ509870. The finding revealed a great potential of the Janthinobacterium lividum strain MMPP4 for biotechnological applications as a source of industrially important enzymes and antimicrobial compound.    

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative