Introduction: Diabetes is a metabolic disease with high prevalence in all over the world. Treatment with synthetic drugs are just to control it and not effective for an absolute cure, thus, they might not be used by the patients at all. By emphasis on traditional medicine and considering the antioxidant property of medicinal plant, Prosopis farcta containing the phenol and flavonoid components, the aim of this study is to evaluate the effect of Prosopis farcta extract on the glucose uptake level in L6 muscle cells line. Materials & Methods: This is an experimental study in which the cells were grown with DMEM medium in appropriate condition. Cells groups treated with and without glucose uptake stimulation, insulin and aqueous and hydroalcoholic extract of Prosopis farcta as well as group by preventing glucose uptake stimulation, Cytochalacin D. To measure the amount of glucose uptake by cells, the instruction of glucose uptake kit (abcam) was used and amount of uptakes was analyzed according to standards. Results: Doses of 160 µg/ml of hydro alcoholic extract and 800 µg/ml of the aqueous extract of Prosopis farcta have the maximum amount of 2- de- oxy glucose uptake in L6 muscle cells (p<0/001) which the dose of 800 µg/ml had the most amount of 2-deoxyglucose uptake in muscle cells (p<0/001). The level of differentiation in L6 muscle cells after FBS reduction is more in comparison with FBS 10% and showing that the muscle cells have better biological activity such as 2-deoxy glucose uptake. Conclusion: Findings showed that both aqueous and hydro alcoholic extract of Prosopis farcta have been effective in reducing the added 2-deoxy glucose of medium and increased the level of glucose uptake. This effect promoted by increasing the extract dose which is concentration dependent manner. However, it is necessary that the accurate role of this plant would be investigated in molecular surveys.
