Introduction: Previous cross-sectional studies have revealed that vitamin D deficiency is associated with prediabetes. Prediabetes increases the risk of type II full diabetes. Considering the significance of diabetes and its high incidence as well as high prevalence of vitamin D deficiency in Iran, more studies need to be carried out in this field to confirm the relationship between prediabetes and vitamin D deficiency. Materials and Methods: It was a case-control study. 208 individuals (55.9%) out of 372 participants were selected as the case group and 164 individuals (44.1%) were selected as the control group based on their fast blood sugar (FBS). The average age of participants was 37.18. Serum 25(OH) D level was compared between the two groups. Findings: No significant difference was observed between serum 25(OH) D of the case group and the control group. Moreover, there was not a significant difference between the two groups regarding their BMIs. Conclusion: No relationship was observed between 25(OH) D and impaired fasting glucose (prediabetes) in individuals with normal FBS. However, it was observed that vitamin D deficiency was quite common among all participants, in such a way that it was observed in 83.6% of all participants. More investigations are recommended to prove the relationship between vitamin D deficiency and prediabetes.