New azo and diazo dye was synthesized by coupling reaction of dizonium salt of P-amino acetophenone, P-phenylene diamine with phenolic derived. The products were characterized by FTIR and 1H NMR; they were identical to literature. A1, A2, A4, A5, B2 and B3 were used dyes in dying process which were applied on wool, cotton and sawdust. We note that there are variations in the hues of the dyed fabrics which is resulted from the modifications in the coupling components. All the dyes gave color changes from light yellow to dark red with good levelness, brightness and depth on the fabrics. The antibacterial activities of the compound were studied and evaluated using gram positive and negative gram stains; A5 and B4 were positive against S. aureas, B1 and B4 were positive against Pseudomonas, and A1, A4, and B4 were positive against E. coli. The purity of the dye was checked by thin layer chromatography (TLC), and also the melting point of the purified dyes was measured.
