Quick access

PDF
Share Article:
2015 Volume 6 Issue 3

Statistical Medium Optimization for the Production of Cephalosporin-C acylase by Pseudomonas diminuta


Abstract
Media composition for Cephalosporin-C acylase (CPC acylase) was optimized using a stepwise strategy. Out of the fifteen nutrients, five key ingredients viz. glucose, beef extract, glutamic acid, adipic acid and sodium chloride were identified for best medium composition by applying Plackett-Burman (PB) statistical design. Results indicated that above factors negatively affects the CPC acylase production i.e. increase in the concentration of these factors decreases the acylase production. The optimal concentration of key ingredients was determined using Central Composite Design (CCD) and the results obtained were fitted with a second order polynomial model. By applying the optimized conditions, a model was designed which predicted the CPC acylase activity as 9.56 U/mL which was in close resemblance to the experimental value of 11.2 U/mL.

INDEXING
ESCI, SCIRUS, BiologyBrowser, Chemical Abstracts, CABI, Intute catalogue, Science Central, EBSCOhost databases, Genamics JournalSeek, Open J gate, Ulrich's, Academic Journals Database, CASSI, ACS (American Chemical Society), CiteFactor, and many other international scientific databases.

JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

Publish with us


Sevas Publishing
Komatipalli Post, Bobbili Mandal, Vizianagaram Dt,
Andhra Pradesh (St), India, Postal Code: 535558

Publishing steps

1.Prepare
your paper
2.Submit
and revise
3.Track
your research
4.Share
and promote
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative