Single strand conformation polymorphism of oligonucleotides in their isolation by means of magnetic micro particles

Studies of biomolecules depend strongly on efficiency of theirisolation from biological materials. For these purposes theapplication of paramagnetic particles (MPs) represents a newmethod. This method, faster and less laborious than commonly used phenol chloroform extraction, is based on the hybridization of target molecules on a surface of magnetic particles. For the isolation of nucleic acids MPs are modified by certain sequences of thymine or other nucleotides. Using MPs we studied the isolation of ODNs (a) with different sequences of adenine and cytosine and (b)with different chain lengths. It was found that single–strand conformation polymorphism (SSCP) of ODNs is crucial phenomena for their isolation.