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2023 Volume 14 Issue 3

Molecular Characterization of Bacterial Isolates Contaminating from Dried Fruits


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Abstract

Producing and processing fruits are impacted by the global issue of fruit safety. Bacteria that might cause serious health issues can infect dried fruits. A total of 180 dried fruit samples including (raisins, dates, and apricots) were purchased from markets in Jeddah Governorate, Saudi Arabia. Morphological bacterial isolation of dried fruits was carried out by adding 5 g of each dried fruit sample to 5 ml of diluent (distilled water). A 0.1 ml volume of each dilution was used to make a series of pour plates. The plates were incubated at 30°C for 48 hr. In addition, the molecular identification analysis of bacterial isolates was confirmed by sequencing the 16S rRNA gene.  The results revealed the isolation of 31 different species belonging to 3 genera of bacteria which are Bacillus, Micrococcus, and Staphylococcus. The most dominant genus is Bacillus sp. (739 CFU) isolated from dried fruits. In conclusion, good manufacturing practices (GMPs) must be applied to maintain healthy dried fruits and reduce dried fruit contamination during and after harvest.


How to cite this article
Vancouver
Alghamdi RG, Qattan SY, Altihani FA, Najjar AA, Noor SO, Makki RM, et al. Molecular Characterization of Bacterial Isolates Contaminating from Dried Fruits. J Biochem Technol. 2023;14(3):72-8. https://doi.org/10.51847/e6nZuI4BPZ
APA
Alghamdi, R. G., Qattan, S. Y., Altihani, F. A., Najjar, A. A., Noor, S. O., Makki, R. M., & Zabermawi, N. M. (2023). Molecular Characterization of Bacterial Isolates Contaminating from Dried Fruits. Journal of Biochemical Technology, 14(3), 72-78. https://doi.org/10.51847/e6nZuI4BPZ
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative