The economic challenge of recombinant pharmaceutical protein production is the separation and purification process. So, optimizing the factors affecting these processes can be considered as an appropriate mechanism for improving productivity. In this research, we can find the temperature and harvest storage time as two important factors affecting the structure and amount of protein produced during the purification process. For this purpose, 7 batches were used. Four batches were kept at -20 ° C and three batches were kept at 4 °C. For this purpose, seven batches were used. The four series were kept at -20 ° C and three series kept at 3 ° C at 4 ° C. Initially, half of the volume of each batch (40 liters) was purified two months after collection, and then the remaining volume was purified after 4 months after collection. Then, the amount of eluted proteins, impurities test, SDS-PAGE and CZE was performed. The purified protein content for the four groups stored at -20 ° C was 0.51, 0.63, 0.46, 0.28 mg/ml and 0.45, 0.58, 0.37, 0.25 mg/ml after 2 and 4 months, respectively. This value was 0.34, 0.32, 0.33 mg/ml and 0.31, 0.27, 0.32 mg/ml after 2 and 4 months for three groups stored at 4 ° C. The CZE test showed that all proteins stored at -20 ° C had a glycosylated structure similar to the standard sample, but the proteins stored at 4 ° C had an incompatibility pattern after four months. The results indicate that improving the conditions of the production process can increase the process efficiency.
