Expressing of bacterial dihydrodipicolinate synthase in transgenic barley

Nutritional quality of human and animal foodstuffs is determined by the content of essential amino acids. Barley is the fourth most important cereal of the world and the second most important cereal grown in the Czech Republic. Cereal grains such as barley contain insufficient levels of some essential amino acid, especially lysine. The ionex chromatography is very selective and convenient for aminoacids determination such as lysine. In connection with post column derivatization the limit of detection of lysine determination was below 1μM and linearity of calibration curve was R2=0.995. Thus we were able to determine the rate of expression for transgenic Barley.