Experimental methods for registration of electrical activity of the heart on cell and whole organ level
Abstract
Modern recording systems for registration of electrical activity ofthe heart are based on application of voltage sensitive dye (VSD)into the examined tissue. Excited VSD's undergo changes in theirelectronic structure, and consequently their fluorescence spectra, inresponse to changes in the surrounding electric field. Fast VSDs areconsistently sensitive probes and can be used in electrophysiologicalstudies. We have proved that optical recording employing di–4–ANEPPS dye utilized in our laboratory is suitable registrationmethod of electrical activity of isolated hearts perfused according toLangendorff. The heart is exposed to VSD diluted in Krebs–Henseleit solution to the concentration of 2μM.
