Controlled drug release is a process in which a nanoparticle specifically incorporated into a medicine is separated from the medicine in a predetermined and desirable manner and the retention time of the drug in the biological system is increased. Therefore, estimation of the lifespan of conjugated nanodrug relative to the retention time of the drug itself is of great importance. In the present study, nanoconjugate release was designed based on RSM statistical method and was tested at different temperatures, pH values, and rotation times. Finally, samples were tested by two methods including high-performance liquid chromatography (HPLC) and absorption concentration reading. The resulting data were analyzed and the drug release stability was calculated in order to obtain the optimum time.
