Background and objective: Helicobacter pylori is considered as a gram-negative, spiral-shaped and microaerophilic bacterium, living in human stomach. This bacterium is often found in patients, who are suffering from chronic gastritis or gastric ulcer. Methodology: marjoram herb was selected for this experimental study. After collecting and preparing the herbs and botanical and pharmacological examinations, the hydro-alcoholic extract of the herbs was prepared using the percolation method and the antimicrobial impacts of this herb on helicobacter pylori was evaluated using disc diffusion method and by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) and by using macro dilution method. Results: research results revealed that hydro-alcoholic extract of marjoram herb had antibacterial impacts on Helicobacter pylori in both methods of macro dilution method and disk diffusion method. The minimum inhibitory concentration of marjoram extract on Helicobacter pylori was found to be 120.25 μg / ml and the minimum bactericidal concentration of this extract was found to be 1100 μg / ml. Conclusion: Marjoram extract can be considered as anti-helicobacter pylori compound. The use of essential oil of this herb at higher concentrations and other methods of essential oil extraction would calrify the anti-Helicobacter pylori impacts of marjoram.
