Determination of metallothionein, glutathione and termostable protein fraction concentrations in tumour tissue from head and neck area

Tumours of head and neck are one of the most incident tumourdiseases. Well-timed diagnosis plays main role in their therapy. Aimof this work consisted in determination of metallothionein (MT),which could be new tumour marker. Determination of itsconcentration was carried out using electrochemical detection withuse of differential pulse voltammetry. Concentrations of MT insamples of denaturized blood plasma of group of patients wereabout 2.3 μM with slight differences in accordance with localizationof tumours. Contents of glutathione and total proteins in denaturizedwere determined chromatographically and by the help of Biuretreaction. Rate of concentrations levels of GSH/GSSG was varyingfrom 1.31 to 17.66.