Determination of GSH and GSSG in blood plasma of patients by HPLCED

Thanks to many chemicals and physical stresses generated byoutside environment there still is unpredictable danger of damaging of organism by reactive oxygen species (ROS). Biological harmfulness of ROS is generally given by the subsequent oxidation of essential cellular structures. Organism is defending against ROS by synthesis of small molecules which can neutralize radicals. The most suitable existing organic molecule widespread in whole organism which can decrease effect of ROS is glutathione (GSH). Therefore we interested in changes of GSH levels by oncological patients. We optimized fast and robust method for analysis blood plasma of patients by employment of HPLC with coulometric detection. Our results based on hunderts of analyzed samples shows significant oxidation damage by disbalance between GSH/GSSG ratio.