Background: Anxiety disorders are one of the most common psychiatric disorders in pregnancy, and this issue can have a negative effect on the fetus. One of the factors intensifying anxiety and depression in pregnant women is diagnostic tests such as amniocentesis. This study has been conducted to investigate the anxiety and depression of women with positive screening result before and after amniocentesis. Methodology: The present study was a descriptive-correlation that was examined 283 amniocentesis candidate pregnant women referred to Al-Zahra Hospital in Tabriz in 2018. Samples were selected using a purpose-based sampling method and entered into the study according to include criteria. The data collection tool included an individual-social questionnaires, and anxiety and depression questionnaire. Questionnaires were completed before and after the result of amniocentesis by pregnant women, and then the collected data was analyzed using statistical paired t-test and SPSS software. Findings: The results showed that, the anxiety rate in of pregnant women before amniocentesis was 91.89 ± 11.52 and 99.68 ± 16.6 after the result of amniocentesis, which had statistically significant difference (p <0.05). In addition, the results of the study showed that the rate of depression in pregnant women before amniocentesis was 26.23 ± 3.33 that after the result of amniocentesis reached 26.26 ± 3.43, which did not have statistically significant difference (p > 0.05). Discussion and Conclusion: The findings showed that, positive results of aneuploidys screening tests, especially Down syndrome, increased anxiety and depression in pregnant women, which had negative effects on mother and fetus, and increased the probability of spontaneous abortion. Therefore, it is suggested that consultations have to take to reduce the anxiety and depression of amniocentesis candidate pregnant women before conducting the procedure through holding educational classes.
